P 191 Corneal endothelial toxicity of liposome encapsulated 5-fluorouridine in rabbits

P"rpse: To investigate the clinical and histological toxicity of the exposure of the :rabbit cornea1 endothelium to anterior chamber injection of liposome-encapsulated 5-fluorouridine (LS-FUR) at concentrations of 1 mg/ml and 0.38 mg/ml and intravitreal injection of I.5FUR at 1 mg/ml. M*thOdS : A total of 12 New Zealand white rabbits were divided in three groups: A) Anterror chamber rnjectian of 0.1 ml Of LS-FUR (1 mg,ml), 8) Lnterior charmer injection Of 0.1 ml "f LS-FUR (0.3R mg,ml), C) Intravitreal injection of LS-FUR (1 mgjml). We treated 3 eyes and 1 cmteol eye (injection of 0.1 ml of empty liposome,s) for each group. We studied the clinical cornea1 edema 24 h after and sacrified the rabbits t3 slcudy the endothelium using light microscopy (s;lver :itain). R..UltS: Treated groups: A) We did nor observe clinical cornea1 edema, hi we demonstrclted a moderate irregular staining of endotheliai membranes. a moderate sign of toxicity. 8) We did not observe clinical edema, but we denwn*teated a lower irregular staining of endotheiial membranes, a moderate sign of toxicity. C) We did not observe ciinlcs.1 edema, and endathelial. membranes were normal suet as the c""trOl group. Conclusions: Anterior chamber injection of liposomr encapsulated 5-fluorouridine (T.-FUR) at concentrations of 1 mg/ml and 0.38 mg/ml causes cyclitic membranes and a maderate histological endotheiium toxicity whereas intravitreal injection of L-SPUR (0.1 mq/ml~ appears nontoxic for the cornea1 endathi~lxum. Purpose : TO identify pos,sible detergents in aqueous humcur, which may cause sillcone oil emulsifxatlon in viva, by means of lli and 1R spectroscopy. Methods: The aqueous humour was aspirated in the beginning of the silicone Oil removal from 35 eyes. ID, 2D NMR and IR spectra of the samples were measured. Results: Albumin, glucose and lactic acid were the best detectable compounds in obtained samples. 1X-l NMR spectroscopy failed to find the other proteins in aqueous humour. Conclusions: IH NMR and IR spectroscopy is a useful method, which can be used for the study of silicone oil emulsification in viva Financial support of the Grant Agency of the Czech Republic, grant No.